Coding

Part:BBa_K1131004:Design

Designed by: Ramya Prathuri   Group: iGEM13_Berkeley   (2013-09-11)


oleD glucosyltransferase; S. antibiotics


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 688
    Illegal NgoMIV site found at 928
    Illegal NgoMIV site found at 1118
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 387
    Illegal BsaI.rc site found at 653
    Illegal BsaI.rc site found at 1078


Design Notes

The sequence has an addition 15 amino acids at the C-terminus when compared to the PDB structure for oleD.


Source

The template was from the Anderson Lab, UC Berkeley, and was mutated by Berkeley iGEM.

References